1 Thuhmahruai
Thau lutuk leh thau lutuk hi khawvel pum huapa hriselna lama harsatna a lo ni ta a, coronary heart disease, hypertension, leh metabolic disorders te nen pawh a inzawm tlat a ni. Pharmaceutical hypolipidemic leh hypoglycemic agents awm tawhte hian side effect an nei fo a, hei hian thisen glucose leh lipids tihregulate nana safe, natural alternatives ngaihvenna a siam a ni.
Seaweed, thei leh thlaiah hian active compound a tam hle a, anti-inflammatory, lipid-lowering, leh hypoglycemic property a nei bawk.Sargassum fusiforme tih a niantioxidant leh weight-management activity a lantir a; curcumin hian lipid metabolism a tidanglam thei a; punicalagin hian anti-inflammatory leh lipid-lowering effect a nei a ni. Heng natural actives zinga -glucosidase leh pancreatic lipase inhibitor te screening hian natural auxiliary hypoglycemic leh hypolipidemic formulations siamna tur lungphum a siam a ni.
2 Thil hman dan leh hman dan
2.1 Thil hman leh Reagent te
Sargassum fusiforme tih a ni, he zirchiannaah hian curcumin, punicalagin, -glucosidase, porcine pancreatic lipase, PNPG, 4-MUO, leh standard inhibitor (acarbose leh orlistat) te hman a ni.
2.2 Hmanraw hman tur
Freeze dryer, rotary evaporator, refrigerated centrifuge, constant-temperature incubator, microplate reader, leh ultrapure water system te hman a ni.
2.3 Experimental tih dan tur
2.3.1 Sargassum fusiforme Tui atanga chhuak siam dan
Hydrated seaweed chu solid–liquid ratio 1:50-ah lakchhuah a ni a, 85 degree-ah 6 h chhung dah a ni a, centrifuged a ni a, freeze-dried a ni a, aqueous extract chu hmuh theih a ni.
2.3.2 -Glucosidase tihkhawtlai dan enfiahna
PNPG colorimetric method hmangin inhibitory activity an zirchiang a.
2.3.3 Pancreatic Lipase tihkhawtlai dan enfiahna
4-MUO chu substrate atan a thawk a, inhibition level chhiar nan fluorescence intensity an teh a ni.
2.3.4 Single-Factor hmanga thil tih dan
Active pathumte IC50 ratio (150:6:2) atanga chhut chuan mass ratio hrang hrang thlan a ni. 0.5 mg/mL total concentration-ah chuan ratio tin atan enzyme pahnih laka inhibition rates an chhut a.
2.3.5 Chhanna lei chunglam design
Three-factor, three-level RSM design chuan ingredient ratio pathumte hian enzyme inhibition a nghawng dan an zirchiang a, optimal composite formula an hmu chhuak a ni.
3 Results leh Analysis te
3.1 IC5 Value tehkhin dan
Punicalagin hian inhibitory activity chak ber a nei a:
-glucosidase hmanga siam a ni.IC5=0.420 mg/mL a ni
Pancreatic lipase hi a awm a: 1.1.IC5=0.090 mg/mL a ni
Curcumin hian activity a nei tlem hle a, chutih laiinSargassum fusiforme tih a niextract chuan inhibition chak lo zawk (IC50=38.22 mg/mL leh 1.02 mg/mL) a lantir.
IC5⁄5 ratio chu a zatve vel a ni150:6:2.
3.2 Single-Factor hmanga thil tih dan
3.2.1 Sargassum fusiforme Extract atanga siam chhuah zat (ratio).
-glucosidase inhibition chu proportion sang zawk nen a tlahniam a, pancreatic lipase inhibition erawh chu ratio 450-ah a peak thung a, optimal ratio thlan chu a ni450.
3.2.2 Curcumin awm zat (ratio).
-glucosidase inhibition chu ratio 4-ah a sang ber a, pancreatic lipase inhibition chu 6-ah a sang ber a, optimal curcumin ratio tihfel chu a ni4.
3.2.3 Punicalagin hman dan (ratio).
Fixed condition (450 Sargassum: 4 curcumin) hnuaiah chuan punicalagin tihpun zauh zauh chuan a chhehvela ratio tha ber hriat theihna a siamsak a ni2, RSM thlirletna atana lungphum siamtu a ni.
3.3 Response Surface thlirletna leh tihchangtlunna
RSM hian enzyme inhibition-a thil tel pathumte nghawng dan chu a hnuaia mi ang hian a tarlang a ni:
-glucosidase tan chuan:
curcumin > punicalagin > Sargassum atanga chhuak a ni
Pancreatic lipase atan chuan:
curcumin > Sargassum atanga chhuak > punicalagin
Model optimization hmangin mass ratio tha ber chu:
150:6:3 (Sargassum atanga lak chhuah : curcumin : punicalagin) tih a ni.
Inhibition result tehna chu:
-glucosidase hmanga siam a ni.47.77%
Pancreatic lipase hi a awm a: 1.1.74.18%
Value te hian model prediction te chu a inmil hle a, model rintlakzia a nemnghet a ni.
3.4 Optimized Formula hmanga Enzyme tihkhawtlai dan
Optimized composite formula hian IC50 value a lantir a, chungte chu:
0.56 mg/mL a ni-glucosidase atan chuan
0.210 mg/mL a nipancreatic lipase atan a ni
Hengte hi an chak zawk tih a chiang hleSargassum fusiforme tih a nialone, combination hnua synergistic enhancement a awm tih a tilang.
4 Sawihona
Active ingredient pathumte hian hypoglycemic leh hypolipidemic effect hriat chhuah an nei vek a, composite formula hian synergistic activity a lantir a ni. Optimized natural formulation hi a him a, thisen glucose leh lipid regulation tumna atana functional foods-a siam theihna chak tak a lantir a ni. Mahse, furtherin vivo-ah a awm bawknakin lawka product siamna atan zirchianna leh regulatory requirement zawm a ngai a ni.
